CD146/MCAM Monoklonaler Antikörper

CD146/MCAM Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, Hund, Kaninchen, Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen(3)

CloneNo.

P1H12

Kat-Nr. : PE-65181

Synonyme

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

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  FC experiment of A375 using PE-65181

  1X10^6 A375 cells were surface stained with 5 ul PE Anti-Human CD146 (PE-65181, Clone: P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

• 

  FC experiment of A375 using PE-65181

  1X10^6 A375 cells were surface stained with 5 ul PE Anti-Human CD146 (PE-65181, Clone: P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: A375-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

PE-65181 bindet in FC CD146/MCAM und zeigt Reaktivität mit human, Hund, Kaninchen, Maus

• Getestete Reaktivität: human, Hund, Kaninchen, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gezüchtete humane Nabelschnurzellen
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 646 aa, 72 kDa
• GenBank-Zugangsnummer: BC056418
• Gene symbol: CD146
• Gene ID (NCBI): 4162
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Publikationen

Species	Application	Title
human	FC	J Dent Sci; N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression.; Authors - Xun Xi
human	FC	J Tissue Eng; Enhanced effects of antagomiR-3074-3p-conjugated PEI-AuNPs on the odontogenic differentiation by targeting FKBP9; Authors - Tao Jiang
		Bioact Mater; Dental pulp stem cells alleviate Schwann cell pyroptosis via mitochondrial transfer to enhance facial nerve regeneration; Authors - Xiaoyu Zheng