CD146/MCAM Polyklonaler Antikörper

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• KD/KO Validated

CD146/MCAM Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17564-1-PBS

Synonyme

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

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  WB analysis using 17564-1-AP (same clone as 17564-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 17564-1-AP (same clone as 17564-1-PBS)

  WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  WB analysis using 17564-1-AP (same clone as 17564-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 17564-1-AP (same clone as 17564-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 17564-1-AP (same clone as 17564-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17564-1-AP (same clone as 17564-1-PBS)

  IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 17564-1-AP (same clone as 17564-1-PBS)

  IP result of anti-CD146/MCAM (IP:17564-1-AP, 3ug; Detection:17564-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 17564-1-AP (same clone as 17564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 17564-1-AP (same clone as 17564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 17564-1-AP (same clone as 17564-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 17564-1-AP (same clone as 17564-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HUVEC using 17564-1-AP (same clone as 17564-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17564-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA CD146/MCAM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD146/MCAM fusion protein Ag11762
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 646 aa, 72 kDa
• Beobachtetes Molekulargewicht: 113-120 kDa
• GenBank-Zugangsnummer: BC056418
• Gene symbol: CD146
• Gene ID (NCBI): 4162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).