• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD146/MCAM Rekombinanter Antikörper

CD146/MCAM Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250806F12

Kat-Nr. : 86159-3-PBS

Synonyme

CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule

Filter:
  • Western Blot (WB) analysis of bEnd.3 cells using CD146/MCAM Recombinant antibody (86159-3-RR)

    WB analysis of bEnd.3 using 86159-3-RR (same clone as 86159-3-PBS)

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 86159-3-RR (Mcam antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86159-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CD146/MCAM Recombinant antibody (86159-3-RR)

    WB analysis of mouse liver using 86159-3-RR (same clone as 86159-3-PBS)

    mouse liver tissue was subjected to SDS PAGE followed by western blot with 86159-3-RR (Mcam antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86159-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using CD146/MCAM Recombinant antibody (86159-3-RR)

    IF Staining of bEnd.3 using 86159-3-RR (same clone as 86159-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD146/MCAM antibody (86159-3-RR, Clone: 250806F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86159-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86159-3-PBS bindet in WB, IF/ICC, Indirect ELISA CD146/MCAM und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD146/MCAM fusion protein Eg2667
Vollständiger Name melanoma cell adhesion molecule
Berechnetes Molekulargewicht72 kDa
Beobachtetes Molekulargewicht120-140 kDa
GenBank-ZugangsnummerNM_023061.2
Gene symbol Mcam
Gene ID (NCBI) 84004
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).