CD146/MCAM Rekombinanter Antikörper

CD146/MCAM Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250806F12

Kat-Nr. : 86159-3-RR

Synonyme

CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule

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  WB analysis of bEnd.3 using 86159-3-RR

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 86159-3-RR (Mcam antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 86159-3-RR

  mouse liver tissue was subjected to SDS PAGE followed by western blot with 86159-3-RR (Mcam antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of bEnd.3 using 86159-3-RR

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD146/MCAM antibody (86159-3-RR, Clone: 250806F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bEnd.3-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: bEnd.3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86159-3-RR bindet in WB, IF/ICC, ELISA CD146/MCAM und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD146/MCAM fusion protein Eg2667
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 120-140 kDa
• GenBank-Zugangsnummer: NM_023061.2
• Gene symbol: Mcam
• Gene ID (NCBI): 84004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).