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CD146/MCAM Rekombinanter Antikörper

CD146/MCAM Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250530B11

Kat-Nr. : 86492-1-PBS

Synonyme

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

Filter:
  • Western Blot (WB) analysis of various lysates using CD146/MCAM Recombinant antibody (86492-1-RR)

    WB analysis using 86492-1-RR (same clone as 86492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86492-1-RR (MCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86492-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CD146/MCAM Recombinant antibody (86492-1-RR)

    IF Staining of HUVEC using 86492-1-RR (same clone as 86492-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using MCAM antibody (86492-1-RR, Clone: 250530B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86492-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86492-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD146/MCAM und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD146/MCAM fusion protein Eg2938
Vollständiger Name melanoma cell adhesion molecule
Berechnetes Molekulargewicht72 kDa
Beobachtetes Molekulargewicht120 kDa
GenBank-ZugangsnummerNM_006500.2
Gene symbol CD146
Gene ID (NCBI) 4162
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).