CD146/MCAM Rekombinanter Antikörper

CD146/MCAM Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250530B11

Kat-Nr. : 86492-1-RR

Synonyme

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

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  WB analysis using 86492-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86492-1-RR (MCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 86492-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using MCAM antibody (86492-1-RR, Clone: 250530B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, L02-Zellen
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86492-1-RR bindet in WB, IF/ICC, ELISA CD146/MCAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD146/MCAM fusion protein Eg2938
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_006500.2
• Gene symbol: CD146
• Gene ID (NCBI): 4162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).