CD146/MCAM Monoklonaler Antikörper
CD146/MCAM Monoklonal Antikörper für
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human
Anwendung
Konjugation
CoraLite®594 Fluorescent Dye
CloneNo.
4D8A9
Kat-Nr. : CL594-66153
Synonyme
Geprüfte Anwendungen
Produktinformation
CL594-66153 bindet in CD146/MCAM und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | CD146/MCAM fusion protein Ag11855 |
| Vollständiger Name | melanoma cell adhesion molecule |
| Berechnetes Molekulargewicht | 646 aa, 72 kDa |
| Beobachtetes Molekulargewicht | 120 kDa |
| GenBank-Zugangsnummer | BC056418 |
| Gene symbol | CD146 |
| Gene ID (NCBI) | 4162 |
| Konjugation | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.
