CD146/MCAM Monoklonaler Antikörper

CD146/MCAM Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF-P

Konjugation

PE Fluorescent Dye

CloneNo.

4D8A9

Kat-Nr. : PE-66153

Synonyme

CD146, 4D8A9, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18

---

• 

  IF Staining of human colon cancer using PE-66153

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE CD146/MCAM antibody (PE-66153, Clone: 4D8A9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using PE-66153

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE CD146/MCAM antibody (PE-66153, Clone: 4D8A9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

PE-66153 bindet in IF-P CD146/MCAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD146/MCAM fusion protein Ag11855
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 646 aa, 72 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC056418
• Gene symbol: CD146
• Gene ID (NCBI): 4162
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.