CD147 Polyklonaler Antikörper

CD147 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32187-1-AP

Synonyme

Basic immunoglobulin superfamily, Basigin, Bsg, HT7 antigen, Membrane glycoprotein gp42

---

• 

  WB analysis using 32187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32187-1-AP (CD147 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Maus-Dünndarmgewebe, Mausmilzgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32187-1-AP bindet in WB, IHC, ELISA CD147 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: basigin
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 35-55 kDa
• GenBank-Zugangsnummer: NM_009768.2
• Gene symbol: CD147
• Gene ID (NCBI): 12215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion, and a single transmembrane region. CD147 is expressed in various cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte, and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported thatthe spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).