CD147 Monoklonaler Antikörper

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• KD/KO Validated

CD147 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G12B5

Kat-Nr. : 66443-1-PBS

Synonyme

1G12B5, 5F7, Basigin, BSG, Collagenase stimulatory factor

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  WB analysis using 66443-1-Ig (same clone as 66443-1-PBS)

  Untreated and PNGase F-treated lysates of TF-1 cells, MOLT-4 cells, Jurkat cells were subjected to SDS PAGE followed by western blot with 66443-1-Ig (CD147 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66443-1-Ig (same clone as 66443-1-PBS)

  WB result of CD147 antibody (66443-1-Ig; 1:40000; room temperature for 1.5 hours) with wild-type and CD147 knockout Jurkat cells. This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 66443-1-Ig (same clone as 66443-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66443-1-Ig (CD147 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50494-1

  Cytometric bead array standard curve of MP50494-1, CD147 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66443-2-PBS. Detection antibody: 66443-1-PBS. Standard:Ag2619. Range: 0.098-3.125 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66443-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA CD147 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD147 fusion protein Ag2619
• Vollständiger Name: basigin (Ok blood group)
• Berechnetes Molekulargewicht: 385 aa, 42 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC009040
• Gene symbol: CD147
• Gene ID (NCBI): 682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).