CD147 Rekombinanter Antikörper

CD147 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241852B4

Kat-Nr. : 98229-1-RR

Synonyme

241852B4, Basic immunoglobulin superfamily, Basigin, Bsg, HT7 antigen

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  FC experiment of mouse thymocytes using 98229-1-RR

  1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse CD147 Rabbit RecAb (98229-1-RR, Clone: 241852B4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse thymocytes using 98229-1-RR

  1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse CD147 Rabbit RecAb (98229-1-RR, Clone: 241852B4) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98229-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98229-1-RR against Mouse CD147 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Thymozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98229-1-RR bindet in FC CD147 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD147 fusion protein Eg1530
• Vollständiger Name: basigin
• Berechnetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_009768.2
• Gene symbol: CD147
• Gene ID (NCBI): 12215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211).