CD151 Polyklonaler Antikörper

CD151 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27816-1-AP

Synonyme

CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1, MER2

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  WB analysis using 27816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27816-1-AP (CD151 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27816-1-AP (CD151 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: unboiled A431 cells, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

27816-1-AP bindet in WB, IHC, ELISA CD151 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD151 fusion protein Ag25968
• Vollständiger Name: CD151 molecule (Raph blood group)
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001374
• Gene symbol: CD151
• Gene ID (NCBI): 977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Publikationen

Species	Application	Title
human	WB,IHC	Sci Rep; Combined knockdown of CD151 and MMP9 may inhibit the malignant biological behaviours of triple-negative breast cancer through the GSK-3β/β-catenin-related pathway; Authors - Fan Li