CD151 Monoklonaler Antikörper

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• KD/KO Validated

CD151 Monoklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2A8G8

Kat-Nr. : 66567-1-Ig

Synonyme

2A8G8, CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1

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  WB analysis using 66567-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 66567-1-Ig

  IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:2000) with human placenta tissue lysate 960 ug.

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  IHC staining of human tonsillitis using 66567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66567-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD151 antibody (66567-1-Ig, Clone: 2A8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: unboiled A431 cells, bei 37°C inkubierte A549-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 3 publications below

Produktinformation

66567-1-Ig bindet in WB, IHC, IF-P, IP, ELISA CD151 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD151 fusion protein Ag25985
• Vollständiger Name: CD151 molecule (Raph blood group)
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC001374
• Gene symbol: CD151
• Gene ID (NCBI): 977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Publikationen

Species	Application	Title
human	WB,IF	Free Radic Biol Med; Downregulation of CD151 induces oxidative stress and apoptosis in trophoblast cells via inhibiting ERK/Nrf2 signaling pathway in preeclampsia.; Authors - Zhiyin Wang ; KD Validated
human	IF	iScience; The proteomic profile of the human myotendinous junction.; Authors - Anders Karlsen
human,mouse	WB	PLoS One; Downregulation of the CD151 protects the cardiac function by the crosstalk between the endothelial cells and cardiomyocytes via exosomes; Authors - Luying Jiang
human	IF	Stem Cells Transl Med; Factor 3 regulates airway engraftment by human bronchial basal cells; Authors - Susan D Reynolds