CD151 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CD151 Monoklonal Antikörper für WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A8G8

Kat-Nr. : 66567-1-PBS

Synonyme

2A8G8, CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1

---

• 

  WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

  IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:2000) with human placenta tissue lysate 960 ug. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD151 antibody (66567-1-Ig, Clone: 2A8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50304-1

  Cytometric bead array standard curve of MP50304-1, CD151 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66567-1-PBS. Detection antibody: 66567-2-PBS. Standard:Ag25985. Range: 0.781-50 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

66567-1-PBS bindet in WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA CD151 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD151 fusion protein Ag25985
• Vollständiger Name: CD151 molecule (Raph blood group)
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC001374
• Gene symbol: CD151
• Gene ID (NCBI): 977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.