CD155/PVR Polyklonaler Antikörper

CD155/PVR Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 31447-1-AP

Synonyme

Pvr, CD155, poliovirus receptor, Tage4

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  WB analysis using 31447-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31447-1-AP (CD155/PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 31447-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31447-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 31447-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31447-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 31447-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31447-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 31447-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31447-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 31447-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD155/PVR antibody (31447-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Mauslebergewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

31447-1-AP bindet in WB, IHC, IF/ICC, ELISA CD155/PVR und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD155/PVR fusion protein Eg0903
• Vollständiger Name: poliovirus receptor
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: NM_027514.2
• Gene symbol: CD155/PVR
• Gene ID (NCBI): 52118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD155, also known as the poliovirus receptor (PVR) or Necl-5, is one of the nectin-like family members, belonging to the Ca2+ independent immunoglobulin superfamily (IgSF) in cell adhesion molecules (PMID: 37660884). CD155 is a type I transmembrane protein composed of three extracellular immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. It is involved in cell-to-cell and cell-to-ECM adhesion (PMID: 32019260; 11437656). CD155 acts as a ligand for immune receptors, including TIGIT, CD96 and CD226, which are expressed on T cells and NK cells. CD155 interacts with these receptors to modulate immune responses, such as T cell activation and NK cell-mediated cytotoxicity (PMID: 37660884). CD155 serves as the entry receptor for poliovirus and thereby mediates susceptibility to poliovirus infection (PMID: 15034010).

Publikationen

Species	Application	Title
mouse,human	WB	Acta Pharm Sin B; PKCα inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability; Authors - Jiaojiao Yu