CD155/PVR Rekombinanter Antikörper

CD155/PVR Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240731B1

Kat-Nr. : 83724-6-RR

Synonyme

Pvr, Tage4, poliovirus receptor, CD155, 240731B1

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  WB analysis using 83724-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83724-6-RR (CD155/PVR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 83724-6-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD155/PVR antibody (83724-6-RR, Clone: 240731B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of RAW 264.7 using 83724-6-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD155/PVR antibody (83724-6-RR, Clone: 240731B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83724-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83724-6-RR against Mouse CD155/PVR were performed. The affinity constant is 0.222 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Mauslebergewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

83724-6-RR bindet in WB, IHC, IF/ICC, ELISA CD155/PVR und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: poliovirus receptor
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: NM_027514.2
• Gene symbol: CD155/PVR
• Gene ID (NCBI): 52118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD155, also known as the poliovirus receptor (PVR) or Necl-5, is one of the nectin-like family members, belonging to the Ca2+ independent immunoglobulin superfamily (IgSF) in cell adhesion molecules (PMID: 37660884). CD155 is a type I transmembrane protein composed of three extracellular immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. It is involved in cell-to-cell and cell-to-ECM adhesion (PMID: 32019260; 11437656). CD155 acts as a ligand for immune receptors, including TIGIT, CD96 and CD226, which are expressed on T cells and NK cells. CD155 interacts with these receptors to modulate immune responses, such as T cell activation and NK cell-mediated cytotoxicity (PMID: 37660884). CD155 serves as the entry receptor for poliovirus and thereby mediates susceptibility to poliovirus infection (PMID: 15034010).

Publikationen

Species	Application	Title
human	WB,IHC	Sci Adv; Ara-C suppresses H3 K27-altered spinal cord diffuse midline glioma growth and enhances immune checkpoint blockade sensitivity; Authors - Bo Pang