CD155/PVR Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

CD155/PVR Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241405F5

Kat-Nr. : 84138-5-RR

Synonyme

PVR, 241405F5, CD155, HVED, Necl 5

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  WB analysis of A549 using 84138-5-RR

  WB result of CD155/PVR antibody (84138-5-RR; 1:1500; room temperature for 1.5 hours) with wild-type and CD155/PVR knockout A549 cells.

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  WB analysis using 84138-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84138-5-RR (CD155/PVR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 84138-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84138-5-RR (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84138-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84138-5-RR (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 84138-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD155/PVR antibody (84138-5-RR, Clone: 241405F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84138-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84138-5-RR against Human CD155/PVR were performed. The affinity constant is 2.95 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, A549-Zellen, HUVEC-Zellen, MKN-45-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84138-5-RR bindet in WB, IHC, IF/ICC, ELISA CD155/PVR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD155/PVR fusion protein Eg0971
• Vollständiger Name: poliovirus receptor
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC015542
• Gene symbol: CD155/PVR
• Gene ID (NCBI): 5817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.