CD155/PVR Monoklonaler Antikörper

CD155/PVR Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

SKII.4

Kat-Nr. : FITC-65251

Synonyme

PVR, CD155, HVED, Necl 5, NECL5

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  FC experiment of U-937 using FITC-65251

  U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul FITC Plus Anti-Human CD155/PVR (FITC-65251, Clone: SKII.4) (red) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21) (blue). Cells were not fixed.

• 

  FC experiment of U-937 using FITC-65251

  U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul FITC Plus Anti-Human CD155/PVR (FITC-65251, Clone: SKII.4) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: U-937-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65251 bindet in FC CD155/PVR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SK-N-AS human neuroblastoma cells
• Vollständiger Name: poliovirus receptor
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC015542
• Gene symbol: CD155/PVR
• Gene ID (NCBI): 5817
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.