CD157 Rekombinanter Antikörper

CD157 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241822H1

Kat-Nr. : 98213-1-RR

Synonyme

Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2

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  FC experiment of mouse splenocytes using 98213-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD157 Rabbit Recombinant Antibody (98213-1-RR, Clone: 241822H1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

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  FC experiment of mouse splenocytes using 98213-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD157 Rabbit Recombinant Antibody (98213-1-RR, Clone: 241822H1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98213-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98213-1-RR against Mouse CD157 were performed. The affinity constant is 0.904 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98213-1-RR bindet in FC CD157 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: bone marrow stromal cell antigen 1
• Berechnetes Molekulargewicht: 35kDa
• Gene symbol: Bst1
• Gene ID (NCBI): 12182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores.