CD16/CD32 Monoklonaler Antikörper

CD16/CD32 Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2a, lambda

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

93

Kat-Nr. : CL488-65057

Synonyme

CD16/32, CD32, 93, Fc gamma receptor IIB, Fc-gamma RII

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  IF Staining of mouse splenocytes using CL488-65057

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD16/32 antibody (CL488-65057, Clone: 93 ) at dilution of 1:100.

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  FC experiment of BALB/c mouse splenocytes using CL488-65057

  1X10^6 BALB/c mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD16/32 (CL488-65057, Clone:93) or 0.5 ug Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65057 bindet in IF/ICC, FC CD16/CD32 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VH81X Tg-B-Zellen und T220-Stromazelllinie
• Vollständiger Name: Fc receptor, IgG, low affinity IIb
• GenBank-Zugangsnummer: BC038070
• Gene symbol: CD16/32
• Gene ID (NCBI): 14130
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.