CD16 Monoklonaler Antikörper

CD16 Monoklonal Antikörper für FC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

3G8

Kat-Nr. : 65090-1-PBS

Synonyme

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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  Sample test of MP50298-3

  Cytometric bead array of Human urine using MP50298-3, CD16a Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-2-PBS. Urine of four individual healthy human donors was analyzed using MP50298-3.

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  Sample test of MP50298-4

  Cytometric bead array of Human urine using MP50298-4, CD16a Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-3-PBS. Urine of four individual healthy human donors was analyzed using MP50298-4.

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  FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50298-3

  Cytometric bead array standard curve of MP50298-3, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-2-PBS. Standard:Eg31662. Range: 1.563-100 ng/mL.

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  Cytometric bead array standard curve of MP50298-4

  Cytometric bead array standard curve of MP50298-4, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-3-PBS. Standard:Eg31662. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

65090-1-PBS bindet in FC, Cytometric bead array, Indirect ELISA, Sample test CD16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC017865
• Gene symbol: CD16a
• Gene ID (NCBI): 2214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).