CD16 Rekombinanter Antikörper

CD16 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242426C2

Kat-Nr. : 98387-1-RR

Synonyme

CD16A, CD16a antigen, CD16-II, Fc gamma RIII, Fc gamma RIII alpha

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  FC experiment of human peripheral blood leukocyte using 98387-1-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2) .or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  FC experiment of human peripheral blood leukocyte using 98387-1-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2)(red).or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG.Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using 98387-1-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2)(red).or Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8 Lot:23015626)(green),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG(red). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98387-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98387-1-RR against Human CD16 were performed. The affinity constant is 12.9 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood leukocyte

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98387-1-RR bindet in FC CD16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD16 fusion protein Eg31662
• Vollständiger Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC017865
• Gene symbol: CD16a
• Gene ID (NCBI): 2214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.