CD16 Rekombinanter Antikörper

CD16 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

3G8

Kat-Nr. : APC-FcA65612

Synonyme

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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  FC experiment of human peripheral blood leukocyte using APC-FcA65612

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody (APC-FcA65612, Clone: 3G8), or 5 ul APC Rabbit IgG Isotype Control Recombinant Antibody (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using APC-FcA65612

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody (APC-FcA65612, Clone:3G8)(red), or 5 ul APC Rabbit IgG Isotype Control Recombinant Antibody (APC-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood leukocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-FcA65612 bindet in FC CD16 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC017865
• Gene symbol: CD16a
• Gene ID (NCBI): 2214
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).