CD16 Rekombinanter Antikörper

CD16 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G8

Kat-Nr. : CL647-65550

Synonyme

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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  FC experiment of human PBMCs using CL647-65550

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD16 Rabbit Recombinant Antibody(CL647-65550, Clone:3G8), and 5 ul FITC Plus Anti-Human CD3 (OKT3) (FITC-65133, Clone: OKT3), and 5 ul CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000). Cells were not fixed. Lymphocytes and monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65550 bindet in FC CD16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC017865
• Gene symbol: CD16a
• Gene ID (NCBI): 2214
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.