CD16 Rekombinanter Antikörper

CD16 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

3G8

Kat-Nr. : FITC-65539

Synonyme

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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  FC experiment of human PBMCs using FITC-65539

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD16 Mouse Recombinant Antibody (FITC-65539, Clone: 3G8) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21), and 5 ul APC Anti-Human CD3 (OKT3) (APC-65133, Clone: OKT3). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65539 bindet in FC CD16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC017865
• Gene symbol: CD16a
• Gene ID (NCBI): 2214
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.