CD16 Rekombinanter Antikörper

CD16 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

3G8

Kat-Nr. : PE-65612

Synonyme

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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  FC experiment of human PBMCs using PE-65612

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3, and 5 ul PE Anti-Human CD16 (3G8) Mouse IgG2a RecAb (PE-65612, Clone: 3G8) or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-65612

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD16 (3G8) Mouse IgG2a RecAb (PE-65612, Clone: 3G8) (red) or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65612 bindet in FC CD16 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC017865
• Gene symbol: CD16a
• Gene ID (NCBI): 2214
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).