- Featured Product
- KD/KO Validated
CD163 Polyklonaler Antikörper
CD163 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human und mehr (2)
Anwendung
WB, IHC, IF-P, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16646-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | humanes Plazenta-Gewebe, Hausschwein-Milzgewebe | 
| Erfolgreiche IP | humanes Plazenta-Gewebe | 
| Erfolgreiche Detektion in IHC | humanes Plazenta-Gewebe,  humanes Lebergewebe,  humanes Lungengewebe,  humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF-P | humanes Tonsillitisgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:1000-1:4000 | 
| Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below | 
| WB | See 47 publications below | 
| IHC | See 89 publications below | 
| IF | See 114 publications below | 
Produktinformation
16646-1-AP bindet in WB, IHC, IF-P, IP, ELISA CD163 und zeigt Reaktivität mit Hausschwein, human
| Getestete Reaktivität | Hausschwein, human | 
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | CD163 fusion protein Ag10029 | 
| Vollständiger Name | CD163 molecule | 
| Berechnetes Molekulargewicht | 1156 aa, 125 kDa | 
| Beobachtetes Molekulargewicht | 130-150 kDa | 
| GenBank-Zugangsnummer | BC051281 | 
| Gene symbol | CD163 | 
| Gene ID (NCBI) | 9332 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for CD163 antibody 16646-1-AP | Protokoll herunterladen | 
| IHC protocol for CD163 antibody 16646-1-AP | Protokoll herunterladenl | 
| IF protocol for CD163 antibody 16646-1-AP | Protokoll herunterladen | 
| IP protocol for CD163 antibody 16646-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease | ||
| Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
| J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages | ||
| Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
| Cell Rep Med Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response | ||
| Nat Commun Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Jaouhara (Verified Customer) (12-05-2023) | Works very well in immunohistochemistry (human cartilage) 
 | 
| FH Kenzo (Verified Customer) (08-29-2023) | Works well by IF on frozen mouse liver tissue sections. 
 | 
| FH Yasuyo (Verified Customer) (05-27-2022) | Works pretty good. 
  | 
| FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue @ 1:100 with a Tris-EDTA antigen retrieval. 
  | 














