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CD163 Polyklonaler Antikörper

CD163 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(230)

Kat-Nr. : 16646-1-AP

Synonyme

CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, Scavenger receptor cysteine-rich type 1 protein M130



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plazenta-Gewebe, Hausschwein-Milzgewebe
Erfolgreiche IPhumanes Plazenta-Gewebe
Erfolgreiche Detektion in IHChumanes Plazenta-Gewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Tonsillitisgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16646-1-AP bindet in WB, IHC, IF-P, IP, ELISA CD163 und zeigt Reaktivität mit Hausschwein, human

Getestete Reaktivität Hausschwein, human
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD163 fusion protein Ag10029
Vollständiger Name CD163 molecule
Berechnetes Molekulargewicht 1156 aa, 125 kDa
Beobachtetes Molekulargewicht 130-150 kDa
GenBank-ZugangsnummerBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CD163 antibody 16646-1-APProtokoll herunterladen
IHC protocol for CD163 antibody 16646-1-APProtokoll herunterladenl
IF protocol for CD163 antibody 16646-1-APProtokoll herunterladen
IP protocol for CD163 antibody 16646-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Mil Med Res

Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease

Authors - Guang-Hui Deng
mouse,humanIF

Nat Aging

Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

Authors - Yifei Cheng
humanIF

J Clin Invest

CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages

Authors - Guohao Zhang
mouseWB

Adv Sci (Weinh)

Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

Authors - Bin Liu
humanIHC

Cell Rep Med

Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response

Authors - Rong Tang
humanIF

Nat Commun

Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target.

Authors - Nourhan Abdelfattah

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jaouhara (Verified Customer) (12-05-2023)

Works very well in immunohistochemistry (human cartilage)

  • Applications: Immunohistochemistry
  • Cell Tissue Type: Cartilage
FH

Kenzo (Verified Customer) (08-29-2023)

Works well by IF on frozen mouse liver tissue sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Liver
FH

Yasuyo (Verified Customer) (05-27-2022)

Works pretty good.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Gastric Cancer
CD163 Antibody Immunofluorescence validation (1:250 dilution) in Gastric Cancer (Cat no:16646-1-AP)
FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue @ 1:100 with a Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Prostate
CD163 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:16646-1-AP)