- Featured Product
- KD/KO Validated
CD163 Polyklonaler Antikörper
CD163 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human und mehr (2)
Anwendung
WB, IHC, IF-P, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16646-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | humanes Plazenta-Gewebe, Hausschwein-Milzgewebe |
Erfolgreiche IP | humanes Plazenta-Gewebe |
Erfolgreiche Detektion in IHC | humanes Plazenta-Gewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | humanes Tonsillitisgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 47 publications below |
IHC | See 89 publications below |
IF | See 114 publications below |
Produktinformation
16646-1-AP bindet in WB, IHC, IF-P, IP, ELISA CD163 und zeigt Reaktivität mit Hausschwein, human
Getestete Reaktivität | Hausschwein, human |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | CD163 fusion protein Ag10029 |
Vollständiger Name | CD163 molecule |
Berechnetes Molekulargewicht | 1156 aa, 125 kDa |
Beobachtetes Molekulargewicht | 130-150 kDa |
GenBank-Zugangsnummer | BC051281 |
Gene symbol | CD163 |
Gene ID (NCBI) | 9332 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for CD163 antibody 16646-1-AP | Protokoll herunterladen |
IHC protocol for CD163 antibody 16646-1-AP | Protokoll herunterladenl |
IF protocol for CD163 antibody 16646-1-AP | Protokoll herunterladen |
IP protocol for CD163 antibody 16646-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease | ||
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
Cell Rep Med Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response | ||
Nat Commun Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jaouhara (Verified Customer) (12-05-2023) | Works very well in immunohistochemistry (human cartilage)
|
FH Kenzo (Verified Customer) (08-29-2023) | Works well by IF on frozen mouse liver tissue sections.
|
FH Yasuyo (Verified Customer) (05-27-2022) | Works pretty good.
![]() |
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue @ 1:100 with a Tris-EDTA antigen retrieval.
![]() |