CD163 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD163 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

130

Rezensionen (4)

Kat-Nr. : 16646-1-AP

Synonyme

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Galerie der Validierungsdaten

• 

  WB analysis of pig spleen using 16646-1-AP

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 16646-1-AP (CD163 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16646-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16646-1-AP (CD163 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16646-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 16646-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD163 antibody (16646-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 16646-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD163 antibody (16646-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver using 16646-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (16646-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver using 16646-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (16646-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 16646-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug CD163 antibody (16646-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Milzgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, humanes Lebergewebe
• Erfolgreiche Detektion in FC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 55 publications below
• IF: See 67 publications below
• FC: See 5 publications below

Produktinformation

16646-1-AP bindet in WB, IHC, IF, FC, ELISA CD163 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD163 fusion protein Ag10029
• Vollständiger Name: CD163 molecule
• Berechnetes Molekulargewicht: 1156 aa, 125 kDa
• Beobachtetes Molekulargewicht: 130-145 kDa
• GenBank-Zugangsnummer: BC051281
• Gene symbol: CD163
• Gene ID (NCBI): 9332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Publikationen

Species	Application	Title
mouse	IF	Mil Med Res; Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease; Authors - Guang-Hui Deng
human	IF	J Clin Invest; CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages; Authors - Guohao Zhang
human	IHC	Cell Rep Med; Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response; Authors - Rong Tang
human	IF	Nat Commun; Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target.; Authors - Nourhan Abdelfattah
human	IHC	EBioMedicine; Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes; Authors - Fan Wu
human	WB,IF	Clin Transl Med; Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour; Authors - Kaiyuan Ji

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jaouhara (Verified Customer) (12-05-2023)

Works very well in immunohistochemistry (human cartilage)

• Applications: Immunohistochemistry
• Cell Tissue Type: Cartilage

FH

Kenzo (Verified Customer) (08-29-2023)

Works well by IF on frozen mouse liver tissue sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Liver

FH

Yasuyo (Verified Customer) (05-27-2022)

Works pretty good.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Gastric Cancer

CD163 Antibody Immunofluorescence validation (1:250 dilution) in Gastric Cancer (Cat no:16646-1-AP)

FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue @ 1:100 with a Tris-EDTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Prostate

CD163 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:16646-1-AP)

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