CD163 Rekombinanter Antikörper

CD163 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240233A3

Kat-Nr. : 83285-4-RR

Synonyme

240233A3, M130, sCD163

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  WB analysis using 83285-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 83285-4-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 83285-4-RR

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using 83285-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat small intestine using 83285-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83285-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83285-4-RR against Mouse CD163 were performed. The affinity constant is 2.25 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mauslungengewebe, Rattenlebergewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenlebergewebe, Ratten-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83285-4-RR bindet in WB, IHC, IF-P, ELISA CD163 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD163 fusion protein Eg0794
• Vollständiger Name: CD163 antigen
• Berechnetes Molekulargewicht: 121 kDa
• Beobachtetes Molekulargewicht: 160-170 kDa
• GenBank-Zugangsnummer: NM_053094.2
• Gene symbol: Cd163
• Gene ID (NCBI): 93671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).