CD163 Monoklonaler Antikörper

CD163 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen(6)

CloneNo.

GHI/61

Kat-Nr. : APC-65169

Synonyme

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

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  FC experiment of human PBMCs using APC-65169

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD14 and 5 ul APC Anti-Human CD163 (APC-65169, Clone: GHI/61), or Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 6 publications below

Produktinformation

APC-65169 bindet in FC CD163 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz
• Vollständiger Name: CD163 molecule
• Berechnetes Molekulargewicht: 1156 aa, 125 kDa
• GenBank-Zugangsnummer: BC051281
• Gene symbol: CD163
• Gene ID (NCBI): 9332
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Publikationen

Species	Application	Title
	FC	Mol Cell Probes; Mesenchymal stem cell-derived exosomes promote tissue repair injury in rats with liver trauma by regulating gut microbiota and metabolism; Authors - Bo Yi
	FC	Eur J Pharmacol; Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK; Authors - Xin Wu
human	FC	Front Immunol; Identification of a distinct cluster of GDF15high macrophages induced by in vitro differentiation exhibiting anti-inflammatory activities; Authors - Chaochao Dai
human	FC	Research (Wash D C); Altered Atlas of Exercise-Responsive MicroRNAs Revealing miR-29a-3p Attacks Armored and Cold Tumors and Boosts Anti-B7-H3 Therapy; Authors - Jie Mei
human	FC	Transl Oncol; Tumor-stroma proportion is associated with increased M2 macrophage abundance and predicts the resistance to immune checkpoint blockade in breast cancer; Authors - Yincheng Liu
human	FC	Life Sci; TPX2 influences the regulation of macrophage polarization via the NF-κB pathway in lung adenocarcinoma; Authors - Lina Wang