CD163 Rekombinanter Antikörper

CD163 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

GHI/61

Kat-Nr. : CL405-65561

Synonyme

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

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  FC experiment of human PBMCs using CL405-65561

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44), and 5 ul CoraLite® Plus 405 Anti-Human CD163 (GHI/61) Mouse IgG2a RecAb (CL405-65561, Clone:GHI/61) or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65561

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD163 (GHI/61) Mouse IgG2a RecAb (CL405-65561, Clone: GHI/61) (red) or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65561 bindet in FC CD163 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz
• Vollständiger Name: CD163 molecule
• Berechnetes Molekulargewicht: 1156 aa, 125 kDa
• GenBank-Zugangsnummer: BC051281
• Gene symbol: CD163
• Gene ID (NCBI): 9332
• Konjugation: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).