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CD163 Polyklonaler Antikörper

CD163 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-16646

Synonyme

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated CD163 Polyclonal ant (CL488-16646)

    IF Staining of human tonsillitis using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated CD163 Polyclonal ant (CL488-16646)

    IF Staining of human tonsillitis using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated CD163 Polyclonal ant (CL488-16646)

    IF Staining of human liver cancer using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646) at dilution of 1:200, CoraLite®555 Beta Catenin antibody (CL555-51067, orange), CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFhumanes Tonsillitisgewebe, humanes Leberkarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-16646 bindet in IF CD163 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD163 fusion protein Ag10029
Vollständiger Name CD163 molecule
Berechnetes Molekulargewicht 1156 aa, 125 kDa
GenBank-ZugangsnummerBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 CD163 antibody CL488-16646Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen