CD163 Monoklonaler Antikörper
CD163 Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
FC
Konjugation
FITC Plus Fluorescent Dye
CloneNo.
GHI/61
Kat-Nr. : FITC-65169
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in FC | humane PBMCs |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| FC | See 2 publications below |
Produktinformation
FITC-65169 bindet in FC CD163 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Maus / IgG1, kappa |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz |
| Vollständiger Name | CD163 molecule |
| Berechnetes Molekulargewicht | 1156 aa, 125 kDa |
| GenBank-Zugangsnummer | BC051281 |
| Gene symbol | CD163 |
| Gene ID (NCBI) | 9332 |
| Konjugation | FITC Plus Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Reinigungsmethode | Mit Protein-A-Affinitätschromatographie gereinigt |
| Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA |
| Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| FC protocol for FITC Plus CD163 antibody FITC-65169 | Download protocol |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Tissue Cell Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization | ||
Discov Oncol Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Julie (Verified Customer) (08-26-2025) | Product used at 5uL per 100uL sample containing approximately 4-5 x 10^5 cells. Only shows slight upregulation in IL-10 treated cells, but literature suggests that this is to be expected. Seemed to work better when cells were not PFA fixed.
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