CD163 Rekombinanter Antikörper

CD163 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

GHI/61

Kat-Nr. : FITC-65522

Synonyme

CD163 molecule, GHI/61, Hemoglobin scavenger receptor, M130, MM130

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  FC experiment of human PBMCs using FITC-65522

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD163 Mouse Recombinant Antibody (FITC-65522, Clone: GHI/61) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21), and 5 ul APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65522

  1x10^6 FC receptor block human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD163 Mouse Recombinant Antibody (FITC-65522, Clone:GHI/61), and 5 ul APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44), and 5 ul FITC Plus Anti-Human CD163 (GHI/61) (FITC-65169, Clone: GHI/61), and FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65522 bindet in FC CD163 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz
• Vollständiger Name: CD163 molecule
• Berechnetes Molekulargewicht: 1156 aa, 125 kDa
• GenBank-Zugangsnummer: BC051281
• Gene symbol: CD163
• Gene ID (NCBI): 9332
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).