CD163 Monoklonaler Antikörper

CD163 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen(6)

CloneNo.

GHI/61

Kat-Nr. : PE-65169

Synonyme

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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  FC experiment of human PBMCs using PE-65169

  1X10^6 human PBMCs were treated with FC receptor block then surface stained with CL405 Anti-Human CD14 and 5 ul PE Anti-Human CD163 (PE-65169, Clone:GHI/61) or PE Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65169

  1X10^6 human PBMCs were treated with FC receptor block then surface stained with 5 ul PE Anti-Human CD163 (PE-65169, Clone:GHI/61) or PE Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 6 publications below

Produktinformation

PE-65169 bindet in FC CD163 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz
• Vollständiger Name: CD163 molecule
• Berechnetes Molekulargewicht: 1156 aa, 125 kDa
• GenBank-Zugangsnummer: BC051281
• Gene symbol: CD163
• Gene ID (NCBI): 9332
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Publikationen

Species	Application	Title
human	FC	Cancer Immunol Immunother; SUMO modified ETV1 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by facilitating CCL2 transcription in esophageal squamous cell carcinoma cells; Authors - Tianci Han
human	FC	Cell Death Discov; Suppression of the hyaluronic acid pathway induces M1 macrophages polarization via STAT1 in glioblastoma.; Authors - Tao Yan
human	FC	J Inflamm Res; Altered Immune Cell Profiles in the Follicular Fluid of Patients with Poor Ovarian Response According to the POSEIDON Criteria; Authors - Ling Zhou
	FC	Cell Mol Biol Lett; LINC00330/CCL2 axis-mediated ESCC TAM reprogramming affects tumor progression; Authors - Lijun Zhao
human	FC	Cell Signal; Single-cell analysis reveals that TCF7L2 facilitates the progression of ccRCC via tumor-associated macrophages; Authors - Fengran Guo
human	FC	Int Immunopharmacol; Radiation induces M2 polarization of glioma-associated macrophages via upregulation of glutamine synthetases; Authors - Yijun Lu