CD164 Rekombinanter Antikörper

CD164 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242986G5

Kat-Nr. : 85723-4-RR

Synonyme

Endolyn, MGC-24, MGC-24v, MUC-24, Multi-glycosylated core protein 24

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  WB analysis of mouse liver using 85723-4-RR

  mouse liver tissue was subjected to SDS PAGE followed by western blot with 85723-4-RR (CD164 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of RM-1 using 85723-4-RR

  Immunofluorescent analysis of (4% PFA) fixed RM-1 cells using CD164 antibody (85723-4-RR, Clone: 242986G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: RM-1 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85723-4-RR bindet in WB, IF/ICC, ELISA CD164 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD164 fusion protein Eg2645
• Vollständiger Name: CD164 antigen
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: NM_016898.2
• Gene symbol: Cd164
• Gene ID (NCBI): 53599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).