CD16a Rekombinanter Antikörper

CD16a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242426G11

Kat-Nr. : 98293-1-PBS

Synonyme

CD16, 242426G11, Fc gamma RIII alpha, Fc gamma RIII, CD16-II

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  FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) , or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98293-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98293-1-RR against Human CD16a were performed. The affinity constant is 14.3 nM.

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Geprüfte Anwendungen

Produktinformation

98293-1-PBS bindet in FC CD16a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Gene symbol: CD16a
• Gene ID (NCBI): 2214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.