CD16b Rekombinanter Antikörper

CD16b Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241713E3

Kat-Nr. : CL647-98192

Synonyme

CD16, FCGR3B, 241713E3, Fc gamma RIII, Fc gamma RIII beta

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  FC experiment of human peripheral blood leukocytes using CL647-98192

  1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD16b Rabbit RecAb (CL647-98192, Clone: 241713E3) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL647-98192

  1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD16b Rabbit RecAb (CL647-98192, Clone: 241713E3) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood leukocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98192 bindet in FC CD16b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD16b fusion protein Eg2487
• Vollständiger Name: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• Gene symbol: FCGR3B
• Gene ID (NCBI): 2215
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).