CD178 (Fas ligand) Rekombinanter Antikörper

CD178 (Fas ligand) Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

NOK-1

Kat-Nr. : CL647-65598

Synonyme

CD178, Fas ligand, FASLG, ADAM10-processed FasL form, Apoptosis antigen ligand

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  FC experiment of Transfected HEK-293T using CL647-65598

  1x10^6 HEK-293T cells or CD178 transfected HEK-293T cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a RecAb (CL647-65598, Clone: NOK-1). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using CL647-65598

  1x10^6 HEK-293T cells (left) or CD178 transfected HEK-293T cells (right) were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a RecAb (CL647-65598, Clone: NOK-1) (red) or 0.25 ug CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65598 bindet in FC CD178 (Fas ligand) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: L5178Y mouse T lymphoma cells expressing recombinant human FasL
• Vollständiger Name: Fas ligand (TNF superfamily, member 6)
• Berechnetes Molekulargewicht: 281 aa, 31 kDa
• GenBank-Zugangsnummer: BC017502
• Gene symbol: Fas Ligand
• Gene ID (NCBI): 356
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD178, also known as Fas ligand, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. It is expressed on NK cells, cytotoxic T lymphocytes and activated CD4+ Th1 cells. CD178 transduces apoptotic signal into cells by binding to FAS/CD95. It is involved as a death factor in the regulation of activation-induced cell death, establishment of immune privilege and tumor cell survival.