CD18 Polyklonaler Antikörper

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• KD/KO Validated

CD18 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Kat-Nr. : 10554-1-AP

Synonyme

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

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Galerie der Validierungsdaten

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  WB analysis using 10554-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10554-1-AP (CD18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10554-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10554-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 10554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 10554-1-AP (CD18 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 10554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 10554-1-AP (CD18 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of RAW 264.7 using 10554-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug CD18 antibody (10554-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• FC: See 2 publications below

Produktinformation

10554-1-AP bindet in WB, IHC, IF, FC, ELISA CD18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD18 fusion protein Ag0861
• Vollständiger Name: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC005861
• Gene symbol: ITGB2
• Gene ID (NCBI): 3689
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Publikationen

Species	Application	Title
human	FC	J Exp Med; Layilin augments integrin activation to promote antitumor immunity.; Authors - Kelly M Mahuron
pig	IF	Research (Wash D C); Regulating Blood Clot Fibrin Films to Manipulate Biomaterial-Mediated Foreign Body Responses; Authors - Yang Zou
human	WB,IF	ACS Appl Mater Interfaces; Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer.; Authors - Yali Jun
human	WB,IHC,IF	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang ; KD Validated
human	WB	Int J Biol Macromol; Diethylaminoethyl-dextran and monocyte cell membrane coated 1,8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis; Authors - Feng Jiang
mouse	WB	Cardiovasc Res; Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice.; Authors - Li Liu