CD19 Rekombinanter Antikörper

CD19 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

4G7

Kat-Nr. : 65562-1-PBS

Synonyme

4G7, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4

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  FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a RecAb (65562-1-MR, Clone: 4G7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65562-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a RecAb (65562-1-MR, Clone: 4G7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65562-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65562-1-PBS bindet in FC CD19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane chronische lymphatische Leukämie (CLL) -Zellen
• Vollständiger Name: CD19 molecule
• Berechnetes Molekulargewicht: 556 aa, 61 kDa
• GenBank-Zugangsnummer: BC006338
• Gene symbol: CD19
• Gene ID (NCBI): 930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. Stable for one year after shipment.

Hintergrundinformationen

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).