CD19 Rekombinanter Antikörper

CD19 Rekombinant Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

1D3

Kat-Nr. : 65661-1-PBS

Synonyme

CD19 antigen

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  FC experiment of mouse splenocytes using 65661-1-TR (same clone as 65661-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone:1D3) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 65661-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 65661-1-TR (same clone as 65661-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone: 1D3) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65661-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65661-1-PBS bindet in FC CD19 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD19 antigen
• GenBank-Zugangsnummer: BC156767
• Gene symbol: Cd19
• Gene ID (NCBI): 12478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).