CD19 Rekombinanter Antikörper

CD19 Rekombinant Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

1D3

Kat-Nr. : 65661-1-TR

Synonyme

CD19 antigen

---

• 

  FC experiment of mouse splenocytes using 65661-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone:1D3) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65661-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone: 1D3) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65661-1-TR bindet in FC CD19 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD19 antigen
• GenBank-Zugangsnummer: BC156767
• Gene symbol: Cd19
• Gene ID (NCBI): 12478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).