CD19 Monoklonaler Antikörper

CD19 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1C10A1

Kat-Nr. : 66298-1-Ig

Synonyme

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Galerie der Validierungsdaten

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  WB analysis of Raji using 66298-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66298-1-Ig

  Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of Ramos using 66298-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66298-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 66298-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (66298-1-Ig, Clone: 1C10A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Raji using 66298-1-Ig

  1X10^6 Raji cells were stained with 0.20ug CD19 antibody (66298-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Raji-Zellen
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:750-1:3000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

66298-1-Ig bindet in WB, IHC, IF, FC, ELISA CD19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD19 fusion protein Ag19945
• Vollständiger Name: CD19 molecule
• Berechnetes Molekulargewicht: 556 aa, 61 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC006338
• Gene symbol: CD19
• Gene ID (NCBI): 930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

Publikationen

Species	Application	Title
human	WB	Cancer Lett; Adenovirus-mediated specific tumor tagging facilitates CAR-T therapy against antigen-mismatched solid tumors.; Authors - Xinying Tang
human	IHC	Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
human	IHC	Int Immunopharmacol; The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data; Authors - Xiaomin Lu
human	WB	In Vitro Cell Dev Biol Anim; The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages.; Authors - Liqiong Chen
human	IF	J Cell Physiol; Upregulation of ENKD1 disrupts cellular homeostasis to promote lymphoma development; Authors - Ting Song
human	IHC,IF	Front Med (Lausanne); Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL; Authors - Xujuan Zhang