CD19 Monoklonaler Antikörper
CD19 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C10A1
Kat-Nr. : 66298-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Raji-Zellen, Daudi-Zellen, Ramos-Zellen |
| Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | Raji-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 2 publications below |
| IHC | See 7 publications below |
| IF | See 4 publications below |
Produktinformation
66298-1-Ig bindet in WB, IHC, IF/ICC, ELISA CD19 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | CD19 fusion protein Ag19945 |
| Vollständiger Name | CD19 molecule |
| Berechnetes Molekulargewicht | 556 aa, 61 kDa |
| Beobachtetes Molekulargewicht | 95 kDa |
| GenBank-Zugangsnummer | BC006338 |
| Gene symbol | CD19 |
| Gene ID (NCBI) | 930 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for CD19 antibody 66298-1-Ig | Protokoll herunterladen |
| IHC protocol for CD19 antibody 66298-1-Ig | Protokoll herunterladenl |
| IF protocol for CD19 antibody 66298-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cancer Lett Adenovirus-mediated specific tumor tagging facilitates CAR-T therapy against antigen-mismatched solid tumors. | ||
Cancer Cell Int Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis. | ||
Int Immunopharmacol The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data | ||
In Vitro Cell Dev Biol Anim The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages. | ||
J Cell Physiol Upregulation of ENKD1 disrupts cellular homeostasis to promote lymphoma development | ||
Front Med (Lausanne) Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL |








