CD19 Rekombinanter Antikörper

CD19 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240135A3

Kat-Nr. : 83254-1-PBS

Synonyme

240135A3, B-lymphocyte antigen CD19, CD19 antigen

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  WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD19 antibody (83254-1-RR, Clone: 240135A3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83254-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

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Geprüfte Anwendungen

Produktinformation

83254-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA CD19 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD19 fusion protein Eg0907
• Vollständiger Name: CD19 antigen
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 62-70 kDa
• GenBank-Zugangsnummer: NM_009844.2
• Gene symbol: Cd19
• Gene ID (NCBI): 12478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).