CD19 Rekombinanter Antikörper

CD19 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242301B9

Kat-Nr. : 84848-5-RR

Synonyme

242301B9, B-lymphocyte antigen CD19, CD19 antigen

---

• 

  WB analysis of mouse splenocytes using 84848-5-RR

  mouse splenocytes were subjected to SDS PAGE followed by western blot with 84848-5-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 84848-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84848-5-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 84848-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84848-5-RR (CD19 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84848-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84848-5-RR against Mouse CD19 were performed. The affinity constant is 6.90 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Splenozyten
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84848-5-RR bindet in WB, IHC, ELISA CD19 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD19 fusion protein Eg3194
• Vollständiger Name: CD19 antigen
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 80-120 kDa
• GenBank-Zugangsnummer: NM_009844.2
• Gene symbol: Cd19
• Gene ID (NCBI): 12478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).