CD19 Monoklonaler Antikörper

CD19 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Atlantic Blue™ Fluorescent Dye

CloneNo.

4G7

Kat-Nr. : AB-65197

Synonyme

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12



Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

AB-65197 bindet in FC CD19 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Humane chronische lymphatische Leukämie (CLL) -Zellen
Vollständiger Name CD19 molecule
Berechnetes Molekulargewicht 556 aa, 61 kDa
GenBank-ZugangsnummerBC006338
Gene symbol CD19
Gene ID (NCBI) 930
Konjugation Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protokolle

Produktspezifische Protokolle
FC protocol for Atlantic Blue™ CD19 antibody AB-65197Protokoll herunterladen
Standard-Protokolle
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