CD19 Rekombinanter Antikörper

CD19 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

4G7

Kat-Nr. : Biotin-65562

Synonyme

4G7, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4

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  FC experiment of human PBMCs using Biotin-65562

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD19 (4G7) Mouse IgG2a RecAb (Biotin-65562, Clone:4G7) and PE-conjugated streptavidin, or unstained. Cells were co-stained with 5 ul CoraLite® Plus 647 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CL647-65570, Clone: UCHT1). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65562

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD19 (4G7) Mouse IgG2a RecAb (Biotin-65562, Clone:4G7) and PE-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-65562 bindet in FC CD19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane chronische lymphatische Leukämie (CLL) -Zellen
• Vollständiger Name: CD19 molecule
• Berechnetes Molekulargewicht: 556 aa, 61 kDa
• GenBank-Zugangsnummer: BC006338
• Gene symbol: CD19
• Gene ID (NCBI): 930
• Konjugation: Biotin
• Excitation/Emission maxima wavelengths: -
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).