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CD1d Monoklonaler Antikörper

CD1d Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1C12F1

Kat-Nr. : 66257-1-Ig

Synonyme

CD1A, CD1D, CD1d molecule, R3, R3G1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of Jurkat cells using CD1d Monoclonal antibody (66257-1-Ig)

    WB analysis of Jurkat using 66257-1-Ig

    WB result of CD1D antibody (66257-1-Ig, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

  • Western Blot (WB) analysis of human testis tissue using CD1d Monoclonal antibody (66257-1-Ig)

    WB analysis of human testis using 66257-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66257-1-Ig (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human small intestine using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human small intestine using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Hodengewebe, Jurkat
Erfolgreiche Detektion in IHChumanes Hautgewebe, humanes Dünndarmgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66257-1-Ig bindet in WB, IHC,ELISA CD1d und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CD1d fusion protein Ag11108
Vollständiger Name CD1d molecule
Berechnetes Molekulargewicht 335 aa, 38 kDa
Beobachtetes Molekulargewicht 40 kDa, 50-55 kDa
GenBank-ZugangsnummerBC027926
Gene symbol CD1D
Gene ID (NCBI) 912
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

Protokolle

Produktspezifische Protokolle
WB protocol for CD1d antibody 66257-1-IgProtokoll herunterladen
IHC protocol for CD1d antibody 66257-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen