CD1d Monoklonaler Antikörper

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• KD/KO Validated

CD1d Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA, IF

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1C12F1

Kat-Nr. : 66257-1-Ig

Synonyme

CD1A, CD1D, CD1d molecule, R3, R3G1

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  WB analysis of Jurkat using 66257-1-Ig

  WB result of CD1D antibody (66257-1-Ig, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

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  WB analysis of human testis using 66257-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66257-1-Ig (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 66257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 66257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human small intestine using 66257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human small intestine using 66257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Jurkat
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

66257-1-Ig bindet in WB, IHC, ELISA, IF CD1d und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD1d fusion protein Ag11108
• Vollständiger Name: CD1d molecule
• Berechnetes Molekulargewicht: 335 aa, 38 kDa
• Beobachtetes Molekulargewicht: 40 kDa, 50-55 kDa
• GenBank-Zugangsnummer: BC027926
• Gene symbol: CD1D
• Gene ID (NCBI): 912
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Open Med (Wars); CD1d affects the proliferation, migration, and apoptosis of human papillary thyroid carcinoma TPC-1 cells via regulating MAPK/NF-κB signaling pathway; Authors - Qingyuan Liu ; KD Validated