CD2 Monoklonaler Antikörper

CD2 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A10B2

Kat-Nr. : 60005-1-PBS

Synonyme

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

---

• 

  WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

  Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 60005-1-Ig (same clone as 60005-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51114-3

  Cytometric bead array standard curve of MP51114-3, CD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60005-1-PBS. Detection antibody: 60781-4-PBS. Standard:Eg0138. Range: 6.25-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

60005-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA CD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD2 fusion protein Ag0423
• Vollständiger Name: CD2 molecule
• Berechnetes Molekulargewicht: 351 aa, 39 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC033583
• Gene symbol: CD2
• Gene ID (NCBI): 914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.