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CD2 Rekombinanter Antikörper

CD2 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241222E10

Kat-Nr. : 84108-3-RR

Synonyme

241222E10, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD2 Recombinant antibody (84108-3-RR)

    IF Staining of Jurkat using 84108-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD2 antibody (84108-3-RR, Clone: 241222E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84108-3-RR

    Affinity and Kinetic Characterization of 84108-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84108-3-RR against Human CD2 were performed. The affinity constant is 4.93 nM.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

84108-3-RR bindet in IF/ICC, ELISA CD2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD2 fusion protein Eg1271
Vollständiger Name CD2 molecule
Berechnetes Molekulargewicht 351 aa, 39 kDa
GenBank-ZugangsnummerBC033583
Gene symbol CD2
Gene ID (NCBI) 914
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CD2 antibody 84108-3-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
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